Review



reverse transcription reaction  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    New England Biolabs reverse transcription reaction
    a. Schematic representation of two transcriptional phenotypes used in this study. Mycophenolic acid (MPA) sensitivity: MPA inhibits the yeast inosine monophosphate dehydrogenase (IMPDH) enzymes encoded by IMD3 and IMD4 , leading to GTP depletion. In response, wild-type cells induce IMD2 expression by a TSS shift from an upstream “G” TSSs, which produces nonfunctional cryptic transcripts, to a downstream “A” TSS that yields a functional IMD2 mRNA. Mutants that are unable to shift the usage to the downstream TSS show MPA S (top). Suppression of imd2 Δ ::HIS3 : The IMD2 ORF was replaced with the HIS3 to generate the reporter construct. In the absence of MPA, wild-type cells initiate <t>transcription</t> from upstream TSSs of the IMD2 promoter, producing nonfunctional transcripts and failing to grow on medium lacking histidine (SC-His). Mutants that shift transcription constitutively to the downstream TSS express HIS3 and show a His⁺ phenotype (bottom). b. Schematic of genetic screening by PCR-based random mutagenesis coupled with gap repair/plasmid shuffle to examine tfb3 or tfa1 alleles. c. Initiation phenotypes of select representative novel tfb3 mutants obtained from genetic screening. d. Initiation phenotypes of select representative novel tfa1 mutants obtained from genetic screening. 10-fold serial dilutions of saturated cultures of WT and mutant strains were plated on different phenotyping media. e. Positions of Tfb3 and Tfa1 relative to TFIIH and Pol II in the yeast PIC (PDB 7O4J) ( S chilbach et al . 2021 ). f. Mapping novel tfb3 and tfa1 mutant residues shown as spheres on the cartoon representation of Tfb3 and Tfa1 protein structures from ( e ). Residues are colored according to phenotype: shades of purple indicate increasing MPA S mutant strength; shades of orange indicate increasing His⁺ mutant strength; exhibiting with distinct substitutions conferring MPA S or His⁺ phenotypes are shown in red.
    Reverse Transcription Reaction, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 4509 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription reaction/product/New England Biolabs
    Average 96 stars, based on 4509 article reviews
    reverse transcription reaction - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "RNA polymerase II-TFIIE-TFIIH interface functions in transcription start site selection in Saccharomyces cerevisiae"

    Article Title: RNA polymerase II-TFIIE-TFIIH interface functions in transcription start site selection in Saccharomyces cerevisiae

    Journal: bioRxiv

    doi: 10.1101/2025.11.05.686797

    a. Schematic representation of two transcriptional phenotypes used in this study. Mycophenolic acid (MPA) sensitivity: MPA inhibits the yeast inosine monophosphate dehydrogenase (IMPDH) enzymes encoded by IMD3 and IMD4 , leading to GTP depletion. In response, wild-type cells induce IMD2 expression by a TSS shift from an upstream “G” TSSs, which produces nonfunctional cryptic transcripts, to a downstream “A” TSS that yields a functional IMD2 mRNA. Mutants that are unable to shift the usage to the downstream TSS show MPA S (top). Suppression of imd2 Δ ::HIS3 : The IMD2 ORF was replaced with the HIS3 to generate the reporter construct. In the absence of MPA, wild-type cells initiate transcription from upstream TSSs of the IMD2 promoter, producing nonfunctional transcripts and failing to grow on medium lacking histidine (SC-His). Mutants that shift transcription constitutively to the downstream TSS express HIS3 and show a His⁺ phenotype (bottom). b. Schematic of genetic screening by PCR-based random mutagenesis coupled with gap repair/plasmid shuffle to examine tfb3 or tfa1 alleles. c. Initiation phenotypes of select representative novel tfb3 mutants obtained from genetic screening. d. Initiation phenotypes of select representative novel tfa1 mutants obtained from genetic screening. 10-fold serial dilutions of saturated cultures of WT and mutant strains were plated on different phenotyping media. e. Positions of Tfb3 and Tfa1 relative to TFIIH and Pol II in the yeast PIC (PDB 7O4J) ( S chilbach et al . 2021 ). f. Mapping novel tfb3 and tfa1 mutant residues shown as spheres on the cartoon representation of Tfb3 and Tfa1 protein structures from ( e ). Residues are colored according to phenotype: shades of purple indicate increasing MPA S mutant strength; shades of orange indicate increasing His⁺ mutant strength; exhibiting with distinct substitutions conferring MPA S or His⁺ phenotypes are shown in red.
    Figure Legend Snippet: a. Schematic representation of two transcriptional phenotypes used in this study. Mycophenolic acid (MPA) sensitivity: MPA inhibits the yeast inosine monophosphate dehydrogenase (IMPDH) enzymes encoded by IMD3 and IMD4 , leading to GTP depletion. In response, wild-type cells induce IMD2 expression by a TSS shift from an upstream “G” TSSs, which produces nonfunctional cryptic transcripts, to a downstream “A” TSS that yields a functional IMD2 mRNA. Mutants that are unable to shift the usage to the downstream TSS show MPA S (top). Suppression of imd2 Δ ::HIS3 : The IMD2 ORF was replaced with the HIS3 to generate the reporter construct. In the absence of MPA, wild-type cells initiate transcription from upstream TSSs of the IMD2 promoter, producing nonfunctional transcripts and failing to grow on medium lacking histidine (SC-His). Mutants that shift transcription constitutively to the downstream TSS express HIS3 and show a His⁺ phenotype (bottom). b. Schematic of genetic screening by PCR-based random mutagenesis coupled with gap repair/plasmid shuffle to examine tfb3 or tfa1 alleles. c. Initiation phenotypes of select representative novel tfb3 mutants obtained from genetic screening. d. Initiation phenotypes of select representative novel tfa1 mutants obtained from genetic screening. 10-fold serial dilutions of saturated cultures of WT and mutant strains were plated on different phenotyping media. e. Positions of Tfb3 and Tfa1 relative to TFIIH and Pol II in the yeast PIC (PDB 7O4J) ( S chilbach et al . 2021 ). f. Mapping novel tfb3 and tfa1 mutant residues shown as spheres on the cartoon representation of Tfb3 and Tfa1 protein structures from ( e ). Residues are colored according to phenotype: shades of purple indicate increasing MPA S mutant strength; shades of orange indicate increasing His⁺ mutant strength; exhibiting with distinct substitutions conferring MPA S or His⁺ phenotypes are shown in red.

    Techniques Used: Expressing, Functional Assay, Construct, Mutagenesis, Plasmid Preparation



    Similar Products

    quantitive reverse transcription polymerase chain reaction qrt pcr system  (Roche)
    99
    Roche quantitive reverse transcription polymerase chain reaction qrt pcr system
    Quantitive Reverse Transcription Polymerase Chain Reaction Qrt Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitive reverse transcription polymerase chain reaction qrt pcr system/product/Roche
    Average 99 stars, based on 1 article reviews
    quantitive reverse transcription polymerase chain reaction qrt pcr system - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    reverse transcription polymerase chain reaction rt pcr 11 ccd 18co cells  (ATCC)
    98
    ATCC reverse transcription polymerase chain reaction rt pcr 11 ccd 18co cells
    Reverse Transcription Polymerase Chain Reaction Rt Pcr 11 Ccd 18co Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr 11 ccd 18co cells/product/ATCC
    Average 98 stars, based on 1 article reviews
    reverse transcription polymerase chain reaction rt pcr 11 ccd 18co cells - by Bioz Stars, 2026-03
    98/100 stars
    		  Buy from Supplier
    reverse transcription quantitative polymerase chain reaction pcr assay  (Bio-Rad)
    99
    Bio-Rad reverse transcription quantitative polymerase chain reaction pcr assay
    Reverse Transcription Quantitative Polymerase Chain Reaction Pcr Assay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative polymerase chain reaction pcr assay/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    reverse transcription quantitative polymerase chain reaction pcr assay - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    reverse transcription polymerase chain reaction rt pcr kit  (TaKaRa)
    96
    TaKaRa reverse transcription polymerase chain reaction rt pcr kit
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription polymerase chain reaction rt pcr kit/product/TaKaRa
    Average 96 stars, based on 1 article reviews
    reverse transcription polymerase chain reaction rt pcr kit - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    real time reverse transcription polymerase chain reaction rt pcr  (Bio-Rad)
    99
    Bio-Rad real time reverse transcription polymerase chain reaction rt pcr
    Real Time Reverse Transcription Polymerase Chain Reaction Rt Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time reverse transcription polymerase chain reaction rt pcr/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    real time reverse transcription polymerase chain reaction rt pcr - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    step quantitative reverse transcription polymerase chain reaction qrt pcr sybr green kit q221  (Vazyme Biotech Co)
    99
    Vazyme Biotech Co step quantitative reverse transcription polymerase chain reaction qrt pcr sybr green kit q221
    Step Quantitative Reverse Transcription Polymerase Chain Reaction Qrt Pcr Sybr Green Kit Q221, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/step quantitative reverse transcription polymerase chain reaction qrt pcr sybr green kit q221/product/Vazyme Biotech Co
    Average 99 stars, based on 1 article reviews
    step quantitative reverse transcription polymerase chain reaction qrt pcr sybr green kit q221 - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier
    reverse transcription reaction  (New England Biolabs)
    96
    New England Biolabs reverse transcription reaction
    a. Schematic representation of two transcriptional phenotypes used in this study. Mycophenolic acid (MPA) sensitivity: MPA inhibits the yeast inosine monophosphate dehydrogenase (IMPDH) enzymes encoded by IMD3 and IMD4 , leading to GTP depletion. In response, wild-type cells induce IMD2 expression by a TSS shift from an upstream “G” TSSs, which produces nonfunctional cryptic transcripts, to a downstream “A” TSS that yields a functional IMD2 mRNA. Mutants that are unable to shift the usage to the downstream TSS show MPA S (top). Suppression of imd2 Δ ::HIS3 : The IMD2 ORF was replaced with the HIS3 to generate the reporter construct. In the absence of MPA, wild-type cells initiate <t>transcription</t> from upstream TSSs of the IMD2 promoter, producing nonfunctional transcripts and failing to grow on medium lacking histidine (SC-His). Mutants that shift transcription constitutively to the downstream TSS express HIS3 and show a His⁺ phenotype (bottom). b. Schematic of genetic screening by PCR-based random mutagenesis coupled with gap repair/plasmid shuffle to examine tfb3 or tfa1 alleles. c. Initiation phenotypes of select representative novel tfb3 mutants obtained from genetic screening. d. Initiation phenotypes of select representative novel tfa1 mutants obtained from genetic screening. 10-fold serial dilutions of saturated cultures of WT and mutant strains were plated on different phenotyping media. e. Positions of Tfb3 and Tfa1 relative to TFIIH and Pol II in the yeast PIC (PDB 7O4J) ( S chilbach et al . 2021 ). f. Mapping novel tfb3 and tfa1 mutant residues shown as spheres on the cartoon representation of Tfb3 and Tfa1 protein structures from ( e ). Residues are colored according to phenotype: shades of purple indicate increasing MPA S mutant strength; shades of orange indicate increasing His⁺ mutant strength; exhibiting with distinct substitutions conferring MPA S or His⁺ phenotypes are shown in red.
    Reverse Transcription Reaction, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription reaction/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    reverse transcription reaction - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier
    transcription reactions  (TaKaRa)
    97
    TaKaRa transcription reactions
    a. Schematic representation of two transcriptional phenotypes used in this study. Mycophenolic acid (MPA) sensitivity: MPA inhibits the yeast inosine monophosphate dehydrogenase (IMPDH) enzymes encoded by IMD3 and IMD4 , leading to GTP depletion. In response, wild-type cells induce IMD2 expression by a TSS shift from an upstream “G” TSSs, which produces nonfunctional cryptic transcripts, to a downstream “A” TSS that yields a functional IMD2 mRNA. Mutants that are unable to shift the usage to the downstream TSS show MPA S (top). Suppression of imd2 Δ ::HIS3 : The IMD2 ORF was replaced with the HIS3 to generate the reporter construct. In the absence of MPA, wild-type cells initiate <t>transcription</t> from upstream TSSs of the IMD2 promoter, producing nonfunctional transcripts and failing to grow on medium lacking histidine (SC-His). Mutants that shift transcription constitutively to the downstream TSS express HIS3 and show a His⁺ phenotype (bottom). b. Schematic of genetic screening by PCR-based random mutagenesis coupled with gap repair/plasmid shuffle to examine tfb3 or tfa1 alleles. c. Initiation phenotypes of select representative novel tfb3 mutants obtained from genetic screening. d. Initiation phenotypes of select representative novel tfa1 mutants obtained from genetic screening. 10-fold serial dilutions of saturated cultures of WT and mutant strains were plated on different phenotyping media. e. Positions of Tfb3 and Tfa1 relative to TFIIH and Pol II in the yeast PIC (PDB 7O4J) ( S chilbach et al . 2021 ). f. Mapping novel tfb3 and tfa1 mutant residues shown as spheres on the cartoon representation of Tfb3 and Tfa1 protein structures from ( e ). Residues are colored according to phenotype: shades of purple indicate increasing MPA S mutant strength; shades of orange indicate increasing His⁺ mutant strength; exhibiting with distinct substitutions conferring MPA S or His⁺ phenotypes are shown in red.
    Transcription Reactions, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcription reactions/product/TaKaRa
    Average 97 stars, based on 1 article reviews
    transcription reactions - by Bioz Stars, 2026-03
    97/100 stars
    		  Buy from Supplier
    reverse transcription quantitative polymerase chain reaction  (Bio-Rad)
    99
    Bio-Rad reverse transcription quantitative polymerase chain reaction
    a. Schematic representation of two transcriptional phenotypes used in this study. Mycophenolic acid (MPA) sensitivity: MPA inhibits the yeast inosine monophosphate dehydrogenase (IMPDH) enzymes encoded by IMD3 and IMD4 , leading to GTP depletion. In response, wild-type cells induce IMD2 expression by a TSS shift from an upstream “G” TSSs, which produces nonfunctional cryptic transcripts, to a downstream “A” TSS that yields a functional IMD2 mRNA. Mutants that are unable to shift the usage to the downstream TSS show MPA S (top). Suppression of imd2 Δ ::HIS3 : The IMD2 ORF was replaced with the HIS3 to generate the reporter construct. In the absence of MPA, wild-type cells initiate <t>transcription</t> from upstream TSSs of the IMD2 promoter, producing nonfunctional transcripts and failing to grow on medium lacking histidine (SC-His). Mutants that shift transcription constitutively to the downstream TSS express HIS3 and show a His⁺ phenotype (bottom). b. Schematic of genetic screening by PCR-based random mutagenesis coupled with gap repair/plasmid shuffle to examine tfb3 or tfa1 alleles. c. Initiation phenotypes of select representative novel tfb3 mutants obtained from genetic screening. d. Initiation phenotypes of select representative novel tfa1 mutants obtained from genetic screening. 10-fold serial dilutions of saturated cultures of WT and mutant strains were plated on different phenotyping media. e. Positions of Tfb3 and Tfa1 relative to TFIIH and Pol II in the yeast PIC (PDB 7O4J) ( S chilbach et al . 2021 ). f. Mapping novel tfb3 and tfa1 mutant residues shown as spheres on the cartoon representation of Tfb3 and Tfa1 protein structures from ( e ). Residues are colored according to phenotype: shades of purple indicate increasing MPA S mutant strength; shades of orange indicate increasing His⁺ mutant strength; exhibiting with distinct substitutions conferring MPA S or His⁺ phenotypes are shown in red.
    Reverse Transcription Quantitative Polymerase Chain Reaction, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative polymerase chain reaction/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    reverse transcription quantitative polymerase chain reaction - by Bioz Stars, 2026-03
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    a. Schematic representation of two transcriptional phenotypes used in this study. Mycophenolic acid (MPA) sensitivity: MPA inhibits the yeast inosine monophosphate dehydrogenase (IMPDH) enzymes encoded by IMD3 and IMD4 , leading to GTP depletion. In response, wild-type cells induce IMD2 expression by a TSS shift from an upstream “G” TSSs, which produces nonfunctional cryptic transcripts, to a downstream “A” TSS that yields a functional IMD2 mRNA. Mutants that are unable to shift the usage to the downstream TSS show MPA S (top). Suppression of imd2 Δ ::HIS3 : The IMD2 ORF was replaced with the HIS3 to generate the reporter construct. In the absence of MPA, wild-type cells initiate transcription from upstream TSSs of the IMD2 promoter, producing nonfunctional transcripts and failing to grow on medium lacking histidine (SC-His). Mutants that shift transcription constitutively to the downstream TSS express HIS3 and show a His⁺ phenotype (bottom). b. Schematic of genetic screening by PCR-based random mutagenesis coupled with gap repair/plasmid shuffle to examine tfb3 or tfa1 alleles. c. Initiation phenotypes of select representative novel tfb3 mutants obtained from genetic screening. d. Initiation phenotypes of select representative novel tfa1 mutants obtained from genetic screening. 10-fold serial dilutions of saturated cultures of WT and mutant strains were plated on different phenotyping media. e. Positions of Tfb3 and Tfa1 relative to TFIIH and Pol II in the yeast PIC (PDB 7O4J) ( S chilbach et al . 2021 ). f. Mapping novel tfb3 and tfa1 mutant residues shown as spheres on the cartoon representation of Tfb3 and Tfa1 protein structures from ( e ). Residues are colored according to phenotype: shades of purple indicate increasing MPA S mutant strength; shades of orange indicate increasing His⁺ mutant strength; exhibiting with distinct substitutions conferring MPA S or His⁺ phenotypes are shown in red.

    Journal: bioRxiv

    Article Title: RNA polymerase II-TFIIE-TFIIH interface functions in transcription start site selection in Saccharomyces cerevisiae

    doi: 10.1101/2025.11.05.686797

    Figure Lengend Snippet: a. Schematic representation of two transcriptional phenotypes used in this study. Mycophenolic acid (MPA) sensitivity: MPA inhibits the yeast inosine monophosphate dehydrogenase (IMPDH) enzymes encoded by IMD3 and IMD4 , leading to GTP depletion. In response, wild-type cells induce IMD2 expression by a TSS shift from an upstream “G” TSSs, which produces nonfunctional cryptic transcripts, to a downstream “A” TSS that yields a functional IMD2 mRNA. Mutants that are unable to shift the usage to the downstream TSS show MPA S (top). Suppression of imd2 Δ ::HIS3 : The IMD2 ORF was replaced with the HIS3 to generate the reporter construct. In the absence of MPA, wild-type cells initiate transcription from upstream TSSs of the IMD2 promoter, producing nonfunctional transcripts and failing to grow on medium lacking histidine (SC-His). Mutants that shift transcription constitutively to the downstream TSS express HIS3 and show a His⁺ phenotype (bottom). b. Schematic of genetic screening by PCR-based random mutagenesis coupled with gap repair/plasmid shuffle to examine tfb3 or tfa1 alleles. c. Initiation phenotypes of select representative novel tfb3 mutants obtained from genetic screening. d. Initiation phenotypes of select representative novel tfa1 mutants obtained from genetic screening. 10-fold serial dilutions of saturated cultures of WT and mutant strains were plated on different phenotyping media. e. Positions of Tfb3 and Tfa1 relative to TFIIH and Pol II in the yeast PIC (PDB 7O4J) ( S chilbach et al . 2021 ). f. Mapping novel tfb3 and tfa1 mutant residues shown as spheres on the cartoon representation of Tfb3 and Tfa1 protein structures from ( e ). Residues are colored according to phenotype: shades of purple indicate increasing MPA S mutant strength; shades of orange indicate increasing His⁺ mutant strength; exhibiting with distinct substitutions conferring MPA S or His⁺ phenotypes are shown in red.

    Article Snippet: A reverse transcription reaction was performed by mixing M-MuLV Reverse Transcriptase (NEB, RNase inhibitor (NEB), dNTPs (GE), DTT, and labeled primer with total RNA.

    Techniques: Expressing, Functional Assay, Construct, Mutagenesis, Plasmid Preparation